中文Kayan aikin gano cutar zazzabin aladu na Afirka
Sunan samfur
Kayan aikin gano cutar zazzabin aladu na Afirka (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Wannan kit ɗin yana amfani da hanyar PCR mai kyalli na ainihi don gano DNA naCutar zazzabin aladu ta Afirka (ASFV)a cikin kayan cututtukan nama irin su tonsils, lymph nodes da splin da kayan cututtuka na ruwa kamar maganin rigakafi da jinin alade.Ya dace da ganowa, ganewar asali da bincike na annobaCutar zazzabin aladu ta Afirka.Kit ɗin shine ALL-shirye PCR SYSTEM (Lyophilized), wanda ya ƙunshi DNA ƙarawa enzyme, mayar da martani, takamaiman alƙawari da bincike da ake buƙata don gano PCR mai kyalli.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Abubuwan da aka bayar na ASFV PCR | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid mai ɗauke da takamaiman gutsuttsura ASFV |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
NasihaSaita
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | ASFV Mai Kyau |
| Undet | ASFV Korau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
