Porcine Circovirus nau'in 2 PCR kayan ganowa

Takaitaccen Bayani:

Wannan kit ɗin yana amfani da hanyar PCR mai kyalli na ainihi don gano RNA na Porcine circovirus nau'in 2 (PCV2) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da ƙwayar cuta da kayan cututtukan ruwa kamar alurar riga kafi da jini na.


Cikakken Bayani

Tags samfurin

Sunan samfur

Porcine circovirus nau'in 2 PCR Gane Kit (Lyophilized)

Girman

48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit

Amfani da Niyya

Wannan kit ɗin yana amfani da hanyar PCR mai kyalli na ainihi don gano RNA na Porcine circovirus nau'in 2 (PCV2) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da ƙwayar cuta da kayan cututtukan ruwa kamar alurar riga kafi da jini na.Ya dace da ganowa, ganewar asali da bincike na annoba na Porcine circovirus nau'in 2. Kit ɗin shine ALL-READY PCR SYSTEM (Lyophilized), wanda ya ƙunshi enzyme amplification na DNA, ƙaddamar da amsawa, ƙayyadaddun ƙayyadaddun bayanai da bincike da ake buƙata don gano PCR mai haske. .

Abubuwan Abubuwan Samfur

Abubuwan da aka gyara Kunshin ƙayyadaddun bayanai Sinadarin
PCV2 PCR Mix 1 × kwalban (Lyophilized foda)  50 Gwaji dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase
6 × 0.2ml 8 rijiyar tube tube(Lyophilized) 48 Gwaji
Kyakkyawan Sarrafa 1 * 0.2ml tube (lyophilized)  10 Gwaji

Plasmid ko Pseudovirus PCV2 dauke da takamaiman gutsuttsura

Narkar da mafita 1.5 ml na Cryotube 500ul /
Sarrafa mara kyau 1.5 ml na Cryotube 200ul 0.9% NaCl

Adana & Rayuwar Rayuwa

(1) Za a iya jigilar kayan a cikin zafin jiki.

(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.

(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.

(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.

Kayan aiki

GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.

Tsarin Aiki

a) Sigar kwalba:

1

b) 8 rijiyar tube version:

2

Amplification na PC

Saitin da aka Shawarar

Mataki

Zagayowar

Zazzabi (℃)

Lokaci

Tashar fluorescence

1

1

95

2 min

/

2

40

95

5s

/

60

10s

Tattara FAM fluorescence

* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.

Sakamakon Gwajin Tafsiri

Tashoshi

Fassarar sakamako

Tashar FAM

Ct≤35

Vibrio Parahaemolyticus Mai Kyau

Undet

Vibrio Parahaemolyticus mara kyau

35

Sakamakon tuhuma, sake gwadawa*

*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.


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