中文Pseudorabies virus (gB) PCR kayan ganowa
Sunan samfur
Pseudorabies virus (gB) PCR Detection Kit (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Wannan kit ɗin yana amfani da hanyar PCR mai kyalli na ainihin lokacin don gano RNA na Pseudorabies virus (gB gene) (PRV) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da splin da kayan cututtukan ruwa kamar alluran rigakafi da jinin aladu.Ya dace da ganowa, ganewar asali da bincike na annoba na Pseudorabies virus (gB gene).Kit ɗin shine ALL-shirye PCR SYSTEM(Lyophilized), wanda ya ƙunshi DNA ƙarawa enzyme, dauki dauki, takamaiman firamare da bincike da ake bukata domin fluorescent RT-PCR gano.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| PRV PCR Mix | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus dauke da takamaiman gutsuttsura PRV(gB). |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | PRV(gB) Mai Kyau |
| Undet | PRV(gB) Mara kyau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
