中文Kwayar cuta ta ƙafa da baki RT-PCR Gane Kit
Sunan samfur
Kwayar cuta ta ƙafa da baki ta RT-PCR Gane Kit (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Wannan kit ɗin yana amfani da hanyar RT-PCR mai kyalli na ainihin-lokaci don gano RNA na cutar ƙafa-da-baki (CSFV) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da saifa da kayan cututtukan ruwa kamar alluran rigakafi da jinin aladu.Ya dace da ganowa, ganewar asali da bincike na annoba na cutar ƙafa da baki.Kit ɗin shine ALL-shirye PCR SYSTEM(Lyophilized), wanda ya ƙunshi DNA ƙarawa enzyme, juyi transcriptase, dauki dauki, takamaiman al'amurra da bincike da ake bukata domin fluorescent PCR gano.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Farashin FMD PCR | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid mai ƙunshe da takamaiman guntu FMD |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
NasihaSaita
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | FMD Tabbatacce |
| Undet | FMD Negative |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
