中文E.coli O157:H7 PCR kayan ganowa
Sunan samfur
E.coli O157:H7 Kayan gano PCR (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Escherichia coli O157:H7 (E.coli O157:H7) kwayar cuta ce ta gram-korau wacce ke cikin kwayar halittar Enterobacteriaceae, wacce ke samar da adadin yawan toxin Vero.A asibiti, yakan faru ba zato ba tsammani tare da ciwon ciki mai tsanani da gudawa na ruwa, sannan kuma zawowar jini bayan kwanaki kadan, wanda zai iya haifar da zazzabi ko rashin zazzabi, da kuma mutuwa a lokuta masu tsanani.Wannan kit ɗin ya dace da gano ƙimar Escherichia coli O157: H7 a cikin abinci, samfuran ruwa, feces, amai, ruwa mai haɓaka ƙwayoyin cuta da sauran samfuran ta amfani da ka'idar PCR na ainihi. Lyophilized), wanda ke ƙunshe da enzyme ƙarawa DNA, buffer amsawa, takamaiman abubuwan da ake buƙata da bincike don gano PCR mai haske.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| E.coli O157:H7 PCR Mix | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid mai ɗauke da E.coli O157:H7 takamaiman gutsuttsura |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
NasihaSaita
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | |
| 2 | 40 | 95 | 5s | |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara siginar tashar FAM fluorescence a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | E.coli O157:H7 Mai Kyau |
| Undet | E.coli O157:H7 Korau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
