Kit ɗin Gano PCR Shigella

Takaitaccen Bayani:

Shigella wani nau'in nau'in gram-negative brevis bacilli ne, mallakar cututtukan hanji, kuma mafi yawan kamuwa da cutar dajin bacillary na ɗan adam.


Cikakken Bayani

Tags samfurin

Sunan samfur

Shigella PCR Kit ɗin Ganewa (lyophilized)

Girman

48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit

Amfani da Niyya

Shigella wani nau'in nau'in gram-negative brevis bacilli ne, mallakar cututtukan hanji, kuma mafi yawan kamuwa da cutar dajin bacillary na ɗan adam.Wannan kit ɗin yana amfani da ƙa'idar PCR mai kyalli na ainihi, wanda ya dace da ƙayyadaddun Shigella a cikin abinci, samfuran ruwa, najasa, amai, da ruwa mai wadatarwa.

Abubuwan Abubuwan Samfur

Abubuwan da aka gyara Kunshin ƙayyadaddun bayanai Sinadarin
Farashin PCR 1 × kwalban (Lyophilized foda)  50 Gwaji dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase
6 × 0.2ml 8 rijiyar tube tube(Lyophilized) 48 Gwaji
Kyakkyawan Sarrafa 1 * 0.2ml tube (lyophilized)  10 Gwaji

Plasmid ko Pseudovirus dauke da takamaiman guntu

Narkar da mafita 1.5 ml na Cryotube 500ul /
Sarrafa mara kyau 1.5 ml na Cryotube 100 l 0.9% NaCl

Adana & Rayuwar Rayuwa

(1) Za a iya jigilar kayan a cikin zafin jiki.

(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.

(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.

(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.

Kayan aiki

GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.

Tsarin Aiki

a) Sigar kwalba:

1

b) 8 rijiyar tube version:

2

Amplification na PC

Saitin da aka Shawarar

Mataki

Zagayowar

Zazzabi (℃)

Lokaci

Tashar fluorescence

1

1

95

2 min

/

2

40

95

5s

/

60

10s

Tattara FAM fluorescence

Sakamakon Gwajin Tafsiri

Tashoshi

Fassarar sakamako

Tashar FAM

Ct≤35

Shigella Mai Kyau

Undet

Shigella Negative

35

Sakamakon tuhuma, sake gwadawa*

*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.


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