Norovirus (GⅠ) Kayan Gano RT-PCR

Takaitaccen Bayani:

Ya dace da gano Norovirus (GⅠ) a cikin kifi, danye kayan lambu da 'ya'yan itatuwa, ruwa, feces, amai da sauran samfurori.Ya kamata a aiwatar da hakar acid nucleic ta kayan cirewar acid nucleic ko hanyar pyrolysis kai tsaye bisa ga nau'ikan samfura daban-daban.


Cikakken Bayani

Tags samfurin

Sunan samfur

Norovirus (GⅠ) Kayan Gano RT-PCR (Lyophilized)

Girman

48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit

Amfani da Niyya

Ya dace da gano Norovirus (GⅠ) a cikin kifi, danye kayan lambu da 'ya'yan itatuwa, ruwa, feces, amai da sauran samfurori.Ya kamata a aiwatar da cirewar acid nucleic ta kayan cirewar nucleic acid ko hanyar pyrolysis kai tsaye bisa ga nau'ikan samfura daban-daban.Kit ɗin shine ALL-shirye PCR SYSTEM (Lyophilized), wanda ya ƙunshi DNA amplification enzyme, reverse transcriptase, reaction buffer, takamaiman primers. da bincike da ake buƙata don gano PCR mai kyalli.

Abubuwan Abubuwan Samfur

Abubuwan da aka gyara Kunshin ƙayyadaddun bayanai Sinadarin
Norovirus (GⅠ) PCR Mix 1 × kwalban (Lyophilized foda)  50 Gwaji dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase
6 × 0.2ml 8 rijiyar tube tube(Lyophilized) 48 Gwaji
Kyakkyawan Sarrafa 1 * 0.2ml tube (lyophilized)  10 Gwaji

Plasmid mai ɗauke da Norovirus (GⅠ) takamaiman gutsuttsura

Narkar da mafita 1.5 ml na Cryotube 500ul /
Sarrafa mara kyau 1.5 ml na Cryotube 200ul 0.9% NaCl

Adana & Rayuwar Rayuwa

(1) Za a iya jigilar kayan a cikin zafin jiki.

(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.

(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.

(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.

Kayan aiki

GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.

Tsarin Aiki

a) Sigar kwalba:

1

b) 8 rijiyar tube version:

2

Amplification na PC

NasihaSaita

Mataki Zagayowar Zazzabi (℃) Lokaci Tashar fluorescence
1 1 50 8 min  
2 1 95 2 min  
3 40 95 5s  
60 10s Tattara FAM fluorescence

* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.

Sakamakon Gwajin Tafsiri

Tashoshi

Fassarar sakamako

Tashar FAM

Ct≤35

Norovirus (GⅠ) Mai Kyau

Undet

Norovirus (GⅠ) Mara kyau

35

Sakamakon tuhuma, sake gwadawa*

*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.


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