中文Kit ɗin Gano PCR na Listeria monocytogenes
Sunan samfur
Kit ɗin Gano PCR na Listeria monocytogenes
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Listeria monocytogenes shine microbacterium mai kyau na gram wanda zai iya girma tsakanin 4 ℃ zuwa 45 ℃.Yana daya daga cikin manyan cututtukan da ke barazana ga lafiyar dan adam a cikin abinci mai sanyi.Babban bayyanar cututtuka sune septicemia, meningitis da mononucleosis.Wannan kit ɗin ya dace da gano ƙimar Listeria monocytogenes a cikin abinci, samfuran ruwa, feces, amai, ruwa mai haɓaka ƙwayoyin cuta da sauran samfuran ta amfani da ka'idar PCR mai haske ta ainihin-lokaci. , wanda ya ƙunshi DNA ƙarawa enzyme, juyi transcriptase, mayar da martani, ƙayyadaddun abubuwan farko da bincike da ake buƙata don gano PCR mai haske.
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Listeria monocytogenes PCR Mix | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid mai dauke da takamaiman gutsuttsuran Listeria monocytogenes |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a)
b)
Amplification na PC
NasihaSaita
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 50 | 8 min | |
| 2 | 1 | 95 | 2 min | |
| 3 | 40 | 95 | 5s | |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | Listeria monocytogenes mai kyau |
| Undet | Listeria monocytogenes mara kyau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
