中文Porcine Circovirus nau'in 2 PCR kayan ganowa
Sunan samfur
Porcine circovirus nau'in 2 PCR Gane Kit (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Wannan kit ɗin yana amfani da hanyar PCR mai kyalli na ainihi don gano RNA na Porcine circovirus nau'in 2 (PCV2) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da ƙwayar cuta da kayan cututtukan ruwa kamar alurar riga kafi da jini na.Ya dace da ganowa, ganewar asali da bincike na annoba na Porcine circovirus nau'in 2. Kit ɗin shine ALL-READY PCR SYSTEM (Lyophilized), wanda ya ƙunshi enzyme amplification na DNA, ƙaddamar da amsawa, ƙayyadaddun ƙayyadaddun bayanai da bincike da ake buƙata don gano PCR mai haske. .
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| PCV2 PCR Mix | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus PCV2 dauke da takamaiman gutsuttsura |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Mai Kyau |
| Undet | Vibrio Parahaemolyticus mara kyau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
