中文Porcine haihuwa da na numfashi cuta RT-PCR Gane Kit
Sunan samfur
Porcine haihuwa da na numfashi cuta cuta RT-PCR gano kit (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Wannan kit ɗin yana amfani da hanyar RT-PCR mai kyalli na ainihin lokaci don gano RNA na Porcine reproductive and breath syndrome virus nucleic acid detection kit (PRRSV) a cikin kayan cututtukan nama kamar tonsils, ƙwayoyin lymph da splin da kayan cututtukan ruwa kamar alurar riga kafi da jini. na aladu .Ya dace da ganowa, ganewar asali da bincike na annoba na Porcine Blue Ear Virus.Kit ɗin shine ALL- SHIRYE PCR SYSTEM(Lyophilized), wanda ya ƙunshi juzu'i na jujjuyawar, DNA ƙarawa enzyme, amsawar amsawa, ƙayyadaddun abubuwan ƙira da bincike da ake buƙata don ganowar RT-PCR mai kyalli.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| PBEV PCR Mix | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincika, Reverse Transcriptase, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus dauke da takamaiman gutsuttsura PRRSV |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 200ul | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 48 | 8 min | / |
| 2 | 1 | 95 | 2 min | / |
| 3 | 40 | 95 | 5s | / |
| 60 | 10s | Tattara FAM fluorescence |
* Lura: Za a tattara sigina na tashoshi na fluorescence na FAM a 60 ℃.
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Mai Kyau |
| Undet | Vibrio Parahaemolyticus mara kyau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
