中文Kit ɗin Gano PCR Shigella
Sunan samfur
Shigella PCR Kit ɗin Ganewa (lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Shigella wani nau'in nau'in gram-negative brevis bacilli ne, mallakar cututtukan hanji, kuma mafi yawan kamuwa da cutar dajin bacillary na ɗan adam.Wannan kit ɗin yana amfani da ƙa'idar PCR mai kyalli na ainihi, wanda ya dace da ƙayyadaddun Shigella a cikin abinci, samfuran ruwa, najasa, amai, da ruwa mai wadatarwa.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Farashin PCR | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus dauke da takamaiman guntu |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 100 l | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 5s | / |
| 60 | 10s | Tattara FAM fluorescence |
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | Shigella Mai Kyau |
| Undet | Shigella Negative |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
