中文Staphylococcus aureus PCR Gane Kit
Sunan samfur
Staphylococcus aureus Kayan Gano PCR (lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Staphylococcus aureus na cikin kwayar halittar Staphylococcus ne kuma kwayar cutar gram-tabbatacce ce.Yana da ƙananan ƙwayoyin cuta na ƙwayoyin cuta wanda ke haifar da abinci na kowa wanda zai iya haifar da enterotoxins kuma ya haifar da guba na abinci.Wannan kit ɗin yana amfani da ƙa'idar PCR mai kyalli na ainihin lokaci kuma ya dace da gano ƙimar Staphylococcus aureus a cikin abinci, samfuran ruwa, feces, amai, ruwa mai wadatarwa da sauran samfuran.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Farashin PCR | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus dauke da takamaiman guntu |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 100 l | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Tattara FAM fluorescence |
Sakamakon Gwajin Tafsiri
| Chankal | Fassarar sakamako |
| FAMChankal | |
| Ct≤35 | Staphylococcus aureus M |
| Undet | Staphylococcus aureus Korau |
| 35<Ct≤40 | Abin tuhumasakamako,sake gwadawa* |
*Idanda sakamakon sake gwajin tashar FAMhasCt darajar ≤40kuma yana nuna hali"S”lanƙwan ƙara girman siffar, ana fassara sakamakon a matsayin tabbatacce, in ba haka ba yana da mummunan.
