中文Vibrio parahaemolyticus PCR Gane Kit
Sunan samfur
Vibrio Parahaemolyticus PCR Gane Kit (Lyophilized)
Girman
48 gwaje-gwaje/kit, 50 gwaje-gwaje/kit
Amfani da Niyya
Vibrio Parahemolyticus (wanda aka fi sani da Halophile Vibrio Parahemolyticus) shine Gram-negative polymorphic bacillus ko Vibrio Parahemolyticus.tare da farawa mai tsanani, ciwon ciki, amai, gudawa da stool na ruwa a matsayin babban alamun asibiti.Wannan kit ɗin yana amfani da ka'idar haske mai haske. PCR kuma ya dace da ganewar inganci na Vibrio Parahaemolyticus a cikin abinci, samfurori na ruwa, feces, amai, da ruwa mai wadatarwa.Kit ɗin shine ALL-READY PCR SYSTEM (Lyophilized), wanda ya ƙunshi DNA amplification enzyme, maida martani, ƙayyadaddun al'amurra. da kuma binciken da ake buƙata don ganowar RT-PCR mai kyalli.
Abubuwan Abubuwan Samfur
| Abubuwan da aka gyara | Kunshin | ƙayyadaddun bayanai | Sinadarin |
| Farashin PCR | 1 × kwalban (Lyophilized foda) | 50 Gwaji | dNTPs, MgCl2, Fim, Bincike, Taq DNA polymerase |
| 6 × 0.2ml 8 rijiyar tube tube(Lyophilized) | 48 Gwaji | ||
| Kyakkyawan Sarrafa | 1 * 0.2ml tube (lyophilized) | 10 Gwaji | Plasmid ko Pseudovirus dauke da takamaiman guntu |
| Narkar da mafita | 1.5 ml na Cryotube | 500ul | / |
| Sarrafa mara kyau | 1.5 ml na Cryotube | 100 l | 0.9% NaCl |
Adana & Rayuwar Rayuwa
(1) Za a iya jigilar kayan a cikin zafin jiki.
(2) Rayuwar shiryayye shine watanni 18 a -20 ℃ da watanni 12 a 2 ℃ ~ 30 ℃.
(3)Duba alamar akan kit don kwanan watan samarwa da ranar karewa.
(4) The lyophilized foda version reagent ya kamata a adana a -20 ℃ bayan rushe da kuma maimaita daskare - narke ya zama kasa da 4 sau.
Kayan aiki
GENECHECKER UF-150, UF-300 kayan aikin walƙiya na ainihin lokacin PCR.
Tsarin Aiki
a) Sigar kwalba:
b) 8 rijiyar tube version:
Amplification na PC
Saitin da aka Shawarar
| Mataki | Zagayowar | Zazzabi (℃) | Lokaci | Tashar fluorescence |
| 1 | 1 | 95 | 2 min | / |
| 2 | 40 | 95 | 6s | / |
| 60 | 12s | Tattara FAM fluorescence |
Sakamakon Gwajin Tafsiri
| Tashoshi | Fassarar sakamako |
| Tashar FAM | |
| Ct≤35 | Vibrio Parahaemolyticus Mai Kyau |
| Undet | Vibrio Parahaemolyticus mara kyau |
| 35 | Sakamakon tuhuma, sake gwadawa* |
*Idan sakamakon sake gwadawa na tashar FAM yana da ƙimar Ct ≤40 kuma yana nuna yanayin ƙara girman sifar "S", ana fassara sakamakon a matsayin tabbatacce, in ba haka ba mara kyau.
